Eucalyptus occidentalis is an important and fast growing species in wood farming and economic exploitation for leather, paper, essence extraction and pharmaceutical industries. In this study, micropropagation of this species was investigated by planting seedlings in MS and GD medium. Sterilization, stability, shooting and rooting treatments were applied to samples as randomized completely design and factorial trails. 0.1 % mercuric chloride solution for 1 minute was used as sterilization treatment. The results showed that appropriate proliferation and elongated growth branches of E. occidentalis were observed in MS medium containing BAP, GA3 and IBA hormones with 0.5, 0.15 and 0.01 mgl-1 concentrations with 200 mgl-1 P.V.P, respectively. The best rooting treatments was obtained in MS medium containing 1/2 Nitrate with IBA, in 1 mgl-1 concentration. Rooted seedlings were successfully adapted in greenhouse conditions.
)