Effect of different treatments on Hippophae rhamnoides seed germination in laboratory

Document Type : Research Paper

Abstract

This paper intended to introduce the pioneer and medicinal sea-buckthorn shrubs for the first time. The effects of different treatments on seed germination seeds of these species were studied in the laboratory. Seeds of Qazvin origin were placed in Petri dishes in germinator after anti-infection and pretreatments. Seed treatments including 1) control, 2) temperature, 3) ice water; 4) hot water 5) lime juice 6) gibberellins were sown in a completely randomized design with four 25 seed replicates. At the end of the experiment, indices of germination percentage, mean shoot length, mean root length, coefficient allometry, shoot number, root number, mean day germination, energy of germination, rate of germination and seed vigor index were calculated. Average germination percentage of treatments for control, cold, ice water, hot water, lime juice and gibberellins were 33, 12, 41, 4, 9 and 32, respectively. The result of ANOVA showed that highest percentage of germination percentage, shoot number, root number and seed vigor index were observed in water ice treatment. The highest value of mean shoot length, mean root length, rate of germination and least value of mean day germination were observed by gibberellins treatment. Allometry coefficient in cold treatment showed the highest value and the highest germination energy was seen in control treatment. Lowest traits occurred mostly in the hot water treatment except mean day germination. Further research suggests for Hippophae rhamnoides L. due to drought tolerance, nitrogen fixation effect and its unique pharmacological properties.

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