The aim of this study was to investigate the allelopathy effect of Prosopis juliflora species on emergence, vigority, gemole and plumule length of two important forest species in southern Iran, Hormozgan province. For this purpose, in the summer of 2020, 15 seeds of Acacia oerfota and Acacia tortilis were planted in the soil collected under, margin and four meters from the canopy edge of Prosopis juliflora and also in the soil mixed with leaf powder of Prosopis juliflora (0, 20, 40, 60 and 80 gr/kg leaf powder in soil) and irrigated with distilled water and their emergencewas recorded once every two days. Finally, gemole and plumule lengths of germinated seeds were measured and the variables were analyzed using GLM statistical analysis at 95% level. The results showed that the emergence percentage in A. tortilis species (20.74%) was significantly higher than A. oerfota species. The gemole length of A. oerfota species (5.14 cm) was longer than A. tortilis while in the soil mixed with prosopis leaf powder, the gemole length in A. oerfota was lower (0.53 cm) than it in A. tortilis. The length of gemole and Plumule in the open area with values of 5.88 and 4.34 cm was significantly higher than their values in the soil under the crown of Prosopis juliflora. Emergence percentages (17.5%), vigority (13.40) and the length of plumule (4.22 cm) were lower in the soil mixed with prosopis leaf powder than those in control treatment. The interactions of species×soil position on vigority (4.40), gemole and plumule length (3.11 and 2.48 cm) showed that the two variables of gemole and plumule length were affected by the soil collection site and had its lowest value under the canopy which it was observed in prosopis leaf concentration treatments. The results showed that A. oerfota species was strongly influenced by Prosopis juliflora allelopathy, therefore, it is recommended to prioritize afforestation with native species and, if possible, to avoid the plantation of Prosopis juliflora in the southern sensitive ecosystems.