مجله جنگل ایران

مجله جنگل ایران

Genetic diversity in Quercus petraea Liebl. of North West of Iran based on SCoT markers

نوع مقاله : مقاله پژوهشی

نویسندگان
1 بخش زیست فناوری موسسه تحقیقات جنگلها و مراتع کشور
2 به نژادی و بیوتکنولوژی دانشگاه تبریز
3 دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز
10.22034/ijf.2025.490673.2020
چکیده
The Arasbaran forest is one of the genetic hotspots of the biosphere due to its high species diversity, and the white oak species plays a special role in the composition and diversity of the biological elements of Arasbaran forests. In this regard, it is necessary and important to evaluate the genetic diversity of the identified seed production area of white oak. In this research, the genetic diversity within and between seven different white oak populations, including 49 different trees, was investigated using the SCoT molecular marker. A total of 129 alleles were observed for these markers, and 100% of the produced alleles were polymorphic. The number of alleles varied from 16 to 29, with marker SCoT41 having the highest number at 29 alleles and marker SCoT7 having the lowest number at 16 alleles. Analysis of molecular variance (AMOVA) showed that 90% of the observed genetic diversity was distributed within populations, while 10% occurred among populations, suggesting that genetic variability within populations was more significant than that among populations. Based on the UPGMA dendrogram, individuals were partitioned into six distinct clades, and according to the UPGMA and PCA patterns, geographical proximity has not played an important role in the formation of the genetic structure of these populations. This research has provided initial insight into the genetic diversity within and between different populations of the Arasbaran white oak species. As a result, it is suggested that, by studying other markers, useful information for conservation and management measures for this valuable species can be obtained.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

تنوع ژنتیکی گونه بلوط سفید (Quercus petraea Liebl.) در شمال غرب ایران بر اساس نشانگرهای SCoT

نویسندگان English

Yousef Mohammadi 1
Shiva Gheytaranpour Sehrigh 2
Sajad Ghanbari 3
1 Plant breeding, Research institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
2 Plant Breeding and Biotechnology Department, Faculty of Agriculture, University of Tabriz, Tabriz
3 Department of Forestry, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran
چکیده English

جنگل ارسباران به دلیل تنوع گونه‌ای بالا، یکی از نقاط داغ ژنتیکی زیست‌کره است و گونه بلوط سفید نقش ویژه‌ای در ترکیب و تنوع عناصر زیستی جنگل‌های ارسباران ایفا می‌کند. در این راستا، ارزیابی تنوع ژنتیکی محاوط بذرگیری شناسایی شده بلوط سفید ضروری و مهم است. در این تحقیق، تنوع ژنتیکی درون و بین هفت جمعیت مختلف بلوط سفید شامل ۴۹ درخت مختلف، با استفاده از نشانگر مولکولی SCoT بررسی شد. در مجموع ۱۲۹ آلل برای این نشانگرها مشاهده شد و ۱۰۰ درصد آلل‌های تولید شده، چندشکل بودند. تعداد آلل‌ها از ۱۶ تا ۲۹ متغیر بود که نشانگر SCoT41 با ۲۹ آلل بیشترین تعداد و نشانگر SCoT7 با ۱۶ آلل کمترین تعداد را داشتند. تجزیه واریانس مولکولی (AMOVA) نشان داد که 90 درصد از تنوع ژنتیکی مشاهده شده در درون جمعیت‌ها و 10 درصد در بین جمعیت‌ها توزیع شده است، که نشان می‌دهد تنوع ژنتیکی در درون جمعیت‌ها قابل توجه‌تر از بین جمعیت‌ها است. بر اساس دندروگرام UPGMA، افراد به شش کلاد مجزا تقسیم شدند و طبق الگوهای UPGMA و PCA، نزدیکی جغرافیایی نقش مهمی در شکل‌گیری ساختار ژنتیکی این جمعیت‌ها نداشته است. این تحقیق بینش اولیه‌ای در مورد تنوع ژنتیکی درون و بین جمعیت‌های مختلف گونه بلوط سفید ارسباران ارائه داده است. در نتیجه، پیشنهاد می‌شود با مطالعه سایر نشانگرها، اطلاعات مفیدی برای اقدامات حفاظتی و مدیریتی این گونه ارزشمند جمع‌آوری شود.

کلیدواژه‌ها English

ارسباران
بلوط سفید
نشانگرهای مولکولی
.GenALEx
Alikhani, L., Rahmani, M.S., Shabanian, N., Badakhshan, H., & Khadivi-Khub, A. (2014). Genetic variability and structure of Quercus brantii assessed by ISSR, IRAP and SCoT markers. Gene, 552(1), 176-183. doi: 10.1016/j.gene.2014.09.034
Alikhani, L., Shabanian, N., Badakhshan, H., & Rahmani, M.S. (2016). Genetic diversity assessment of gal oak (Quercus infectoria) populations in north Zagros forests using ScoT molecular marker and morphological and biochemical characteristics. Modern Genetic Journal 11(1), 121-133. doi: 20.1001.1.20084439.1395.11.1.13.6. (In Persian)
Amom, T., Tikendra, L., Apana, N., Goutam, M., Sonia, P., Koijam, A.S., & Nongdam, P. (2020). Efficiency of RAPD, ISSR, iPBS, SCoT and phytochemical markers in the genetic relationship study of five native and economical important bamboos of North-East India. Phytochemistry, 174, 112330. doi: 10.1016/j.phytochem.2020.112330
Aydın, F., Özer, G., Alkan, M., & Çakır, İ. (2022). Start Codon Targeted (SCoT) markers for the assessment of genetic diversity in yeast isolated from Turkish sourdough. Food Microbiology, 107, 104081. doi: 10.1016/j.fm.2022.104081
Banaei-Asl, F., & Mohammadi, Y. (2025). Assessment of genetic diversity among Acer cappadocicum Gled. Elite genotypes using Molecular markers for seed orchard formation. Iranian Journal of Forest, 16(4), 489-501. doi:10.22034/ijf.2024.428309.1956. (In Persian)
Buer, H., Rula, S., Wang, Z.Y., Fang, S., & Bai, Y.E. (2022). Analysis of genetic diversity in Prunus sibirica L. in inner Mongolia using SCoT molecular markers. Genetic Resources and Crop Evolution, 69(3), 1057-1068. doi: 10.1007/s10722-021-01284-4
Burczyk, J., Chybicki, I.J., & Trojankiewicz, M. (2018). High genetic diversity promotes a common-garden trial of Quercus robur as a potential seed source. Dendrobiology, 79, 1-9. doi:10.12657/denbio.079.001
Collard, B.C., & Mackill, D.J. (2009). Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant molecular biology reporter, 27, 86-93. doi: 10.1007/s11105-008-0060-5
Dvořák, J., Korecký, J., Faltinová, Z., & Zádrapová, D. (2022). Genetic diversity of sessile oak populations in the Czech Republic. Journal of Forest Science, 68(1), 8-18. doi: 10.17221/99/2021-JFS
Eaton, E.G.S.D.J., Caudullo, G., Oliveira, S., & De Rigo, D. (2016). Quercus robur and Quercus petraea in Europe: distribution, habitat, usage and threats. European atlas of forest tree species, 160-163.
Ghamari Zare, A., Espahbodi, K., & Mehrabi, A.A. (2023). Seed orchards and seed production areas for forest species are effective strategies for genetic protection, reclamation, and developing forests resistant to climate change. Iran Nature, 8(2), 7-13. doi: 10.22092/irn.2023.129209. (In Persian)
Gogoi, B., Wann, S.B., & Saikia, S.P. (2020). Comparative assessment of ISSR, RAPD, and SCoT markers for genetic diversity in Clerodendrum species of North East India. Molecular Biology Reports, 47, 7365-7377. doi: 10.1007/s11033-020-05792-x
Gömöry, D., Yakovlev, I., Zhelev, P., Jedináková, J., & Paule, L. (2001). Genetic differentiation of oak populations within the Quercus robur/Quercus petraea complex in Central and Eastern Europe. Heredity, 86(5), 557-563. doi: 10.1046/j.1365-2540.2001.00874.x
Hamzeh, B., Safavi, S., Asri, Y., & Jalili, A. (2010). 'Floristic analysis and a preliminary vegetation description of Arasbaran Biosphere Reserve, NW Iran'. Rostaniha, 11(1), 1-16. (In Persian)
Hao, J., Jiao, K., Yu, C., Guo, H., Zhu, Y., Yang, X., & Shen, C. (2018). Development of SCoT-based SCAR marker for rapid authentication of Taxus media. Biochemical genetics, 56, 255-266. doi: 10.1007/s10528-018-9842-0
Hughes, A.R., Inouye, B.D., Johnson, M.T., Underwood, N., & Vellend, M. (2008). Ecological consequences of genetic diversity. Ecology letters, 11(6), 609-623. doi: 10.1111/j.1461- 0248.2008.01179.x
Jensen, J.S., Olrik, D.C., Siegismund, H.R., & Lowe, A.J. (2003). Population genetics and spatial autocorrelation in an unmanaged stand of Quercus petraea in Denmark. Scandinavian Journal of Forest Research, 18(4), 295-304. doi: 10.1080/02827580310005072
Jurkšienė, G., Baranov, O.Y., Kagan, D.I., Kovalevič-Razumova, O.A., & Baliuckas, V. (2020). Genetic diversity and differentiation of pedunculate (Quercus robur) and sessile (Q. petraea) oaks. Journal of Forestry Research, 31(6), 2445-2452. doi: 10.1007/s11676-019-01043-3
Kesić, L., Cseke, K., Orlović, S., Stojanović, D.B., Kostić, S., Benke, A., & Avramidou, E.V. (2021). Genetic diversity and differentiation of pedunculate oak (Quercus robur L.) populations at the Southern Margin of its distribution range Implications for conservation. Diversity, 13(8), 371. doi: 10.3390/d13080371
Khadivi-Khub, A., Shabanian, N., Alikhani, L., & Rahmani, M.S. (2015). Genotypic analysis and population structure of Lebanon oak (Quercus libani G. Olivier) with molecular markers. Tree Genetics & Genomes, 11, 1-10. doi: 10.1007/s11295-015-0935-1
Luo, C., He, X.H., Chen, H., Ou, S.J., & Gao, M.P. (2010). Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers. Biochemical Systematics and Ecology, 38(6), 1176-1184. doi: 10.1016/j.bse.2010.11.004
Mohammadi, Y., Mashayekhi, M.R., & Gheytaranpour Sehrigh, S. (2025). Evaluation of genetic diversity and population structure of Juniperus excelsa M. Bieb. in Ardabil using SCoT markers. Iranian Journal of Forest, 17(1), 1-12. doi: 10.22034/ijf.2024.433673.1960. (In Persian)
Motahari, B., Shabanian, N., Rahmani, M.S., & Mohammad-Hasani, F. (2021). Genetic diversity and genetic structure of Acer monspessulanum L. across Zagros forests of Iran using molecular markers. Gene, 769, 145245. doi: 10.1016/j.gene.2020.145245
Muir, G., Lowe, A.J., Fleming, C.C., & Vogl, C. (2004). High nuclear genetic diversity, high levels of outcrossing and low differentiation among remnant populations of Quercus petraea at the margin of its range in Ireland. Annals of Botany, 93(6), 691-697. doi: 10.1093/aob/mch096
Nagy, S., Poczai, P., Cernák, I., Gorji, A.M., Hegedűs, G., & Taller, J. (2012). PICcalc: an online program to calculate polymorphic information content for molecular genetic studies. Biochemical genetics, 50, 670-672. doi: 10.1007/s10528-012-9509-1
Peakall, R., & Smouse, P.E. (2012). GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics, 28, 2537–2539. doi: 10.1093/ bioinformatics/bts460.
Petit, R.J., Bodénès, C., Ducousso, A., Roussel, G., & Kremer, A. (2004). Hybridization as a mechanism of invasion in oaks. New Phytologist, 161(1), 151-164. doi: 10.1046/j.1469-8137.2003.00944.x
Porth, I., & El-Kassaby, Y.A. (2014). Assessment of the genetic diversity in forest tree populations using molecular markers. Diversity, 6(2), 283-295. doi: 10.3390/d6020283
Rebrean, F.A., Fustos, A., Szabo, K., Lisandru, T.T., Rebrean, M.S., Varga, M.I., & Pamfil, D. (2023). Genetic Diversity and Structure of Quercus petraea (Matt.) Liebl. Populations in Central and Northern Romania Revealed by SRAP Markers. Diversity, 15(10), 1093. doi: 10.3390/d15101093
Rohlf, F.J. (2000). NTSYS 2.1: numerical taxonomic and multivariate analysis system. New York, Exeter Software.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R. (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences, 81(24), 8014-8018. doi: 10.1073/pnas.81.24.8014
Sandurska, E., Ulaszewski, B., & Burczyk, J. (2019). Genetic diversity and differentiation of coexisting populations of Quercus robur L. and Q. petraea (Matt.) Liebl. Acta Biologica Cracoviensia. Series Botanica, 61(1). doi: 10.24425/abcsb.2019.127739
Shabanian, N., Alikhani, L., & Rahmani, M.S. (2015). Phenotypic and genotypic diversity in brant oak (Quercus brantii) populations of declining north-Zagros forests using biochemical characteristics and molecular SCoT marker. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23(1), 13-29. doi:10.22092/ijrfpbgr.2015.101532 (In Persian).
Tóth, E.G., Cseke, K., Benke, A., Lados, B.B., Tomov, V.T., Zhelev, P., & Köbölkuti, Z. A. (2023). Key triggers of adaptive genetic variability of sessile oak (Q. petraea (Matt.) Liebl.) from the Balkan refugia: outlier detection and association of SNP loci from ddRAD-seq data. Heredity, 131(2), 130-144. doi: 1038/s41437-023-00629-2
Vivodík, M., Balážová, Ž., Gálová, Z., & Petrovičová, L. (2019). Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated castor (Ricinus communis L.) genotypes. Genetika, 51(1), 137-146. doi: 10.2298/GENSR1901137V

  • تاریخ دریافت 17 آذر 1403
  • تاریخ بازنگری 13 شهریور 1404
  • تاریخ پذیرش 04 مرداد 1404