القای مقاومت به خشکی و قارچ زغالی در نهال‌های برودار با استفاده از پرایمینگ بذر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد گروه جنگل، مرتع و آبخیزداری، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه یاسوج، یاسوج، ایران

2 دانشیار گروه جنگل، مرتع و آبخیزداری، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه یاسوج، یاسوج، ایران

3 استادیار گروه گیاهپزشکی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه یاسوج، یاسوج، ایران

چکیده

در این تحقیق از پرایمینگ بذر بلوط با مواد مختلف برای القای مقاومت به قارچ زغالی و خشکی در سال اول رشد استفاده شد. به‌ این ‌منظور بذرها در محلول‌هایی از اسید سالیسیلیک، اسید ایندول بوتریک و نیترات پتاسیم خیسانده شده و نهال‌های حاصل از نظر مقاومت به قارچ زغالی بلوط و خشکی آزمایش شدند. تیمارهای کمبود آب (25 درصد ظرفیت زراعی) و مایه‌زنی با قارچ بیماری‌زا (Biscogniauxia mediterranea) موجب کاهش عملکرد فتوسیستم، شاخص کلروفیل، پتانسیل آبی، تعرق و هدایت روزنه‌ای در نهال‌های برودار شدند. پرایمینگ بذرها با اسید سالیسیلیک بیشترین تأثیر را در محدود کردن زخم ساقه در نهال‌های حاصل داشت و در شرایط تنش رطوبتی، پتانسیل آبی ریشه و ساقه را حفظ کرد و فراوانی سلول‌های پارانشیمی اشعه را افزایش داد. پرایمینگ با اسید ایندول بوتریک اثرهای مشابهی را در محدود کردن زخم نشان داد، اما اثرهای ضعیف‌تری نسبت به اسید سالیسیلیک بر روابط آبی گیاه و فراوانی سلول‌های پارانشیمی اشعه نشان داد. نیترات پتاسیم تأثیری در القای مقاومت به بیماری نشان نداد و تغییر چندانی در روابط آبی گیاه ایجاد نکرد. در مجموع به نظر می‌رسد که پرایمینگ بذر با اسید سالیسیلیک تأثیر مهمی در القای مقاومت سیستمیک نهال‌های برودار به قارچ زغالی و خشکی در سال اول رشد دارد. استفاده از نتایج این تحقیق می‌تواند راهکارهای نوینی را برای دستیابی به نهال‌های اصلاح‌شده در اختیار قرار دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Resistance induction to drought and charcoal disease in brant’s oak seedling by seed priming

نویسندگان [English]

  • P Samimi 1
  • P Fayyaz 2
  • F Ghaderi 3
  • R Zolfaghari 2
1 1M.Sc. Student of Forest Range and Watershed Management, Faculty of Agriculture and Natural Resources, University of Yasouj, Yasuj, I.R. Iran
2 Associate Prof., Dept. of Forest Range and Watershed Management, Faculty of Agriculture and Natural Resources, University of Yasouj, Yasuj, I.R. Iran
3 Assistant Prof., Dept. of Plant Protection, Faculty of Agriculture and Natural Resources, University of Yasouj, Yasuj, I.R. Iran
چکیده [English]

This study investigates the use of seed priming with different materials to induce resistance to charcoal disease and drought in the first year of growth of brant's oak. The seeds were soaked in solutions of salicylic acid, indole butyric acid, and potassium nitrate with three different concentrations, and the seedlings were tested for resistance to oak charcoal and drought. Both water deficit treatment (25 percent field capacity) and inoculation with the pathogenic fungus (Biscogniauxia mediterranea) declined photosystem performance, chlorophyll index, water potential, transpiration, and stomatal conductance in brant’s oak seedlings. The results show that priming of seeds with 150 ppm salicylic acid had the greatest effect on limiting stem lesion in the resulting seedlings and maintained the root potential of the root and stem under conditions of moisture stress. Priming with 100 ppm indole butyric acid showed similar effects in limiting the lesion but showed weaker effects than salicylic acid on plant water relationships and the abundance of ray parenchyma cells. Potassium nitrate (0.5 percent) had no effect on disease resistance and did not alter plant water relations. Overall, the study suggests that seed priming with salicylic acid plays an effective role in inducing systemic resistance of seedlings to charcoal disease and drought in the first year. The findings of this study can offer new insights to achieve improved seedlings.

کلیدواژه‌ها [English]

  • Indole butyric acid
  • Salicylic acid
  • Oak charcoal disease
  • Water deficit
  • Potassium nitrate
 
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